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FIVE Tools

MGI WashU - Bioimaging Suite - Current version v0.9.52

Desktop suite for plate-based bioimaging analysis: segmentation, degeneration index, and wavelength tooling. Install once; it updates itself automatically.

Windows

Windows 10/11 - 64-bit - installer
Download for Windows

macOS

Apple Silicon (arm64) - macOS 14+ - installer package
Download for macOS
Auto-updates: after the first install, FIVE Tools checks for new versions on launch and prompts you to update. You will not need to come back here.

Windows - first install

  1. Run the downloaded FiveAvalonia-win-Setup.exe.
  2. If Windows shows "Windows protected your PC", click More info - Run anyway.
  3. It installs to your user profile and launches. Future versions update automatically.

macOS - first install

  1. Run the downloaded FiveAvalonia-osx-Setup.pkg.
  2. If macOS blocks the installer, right-click it and choose Open.
  3. It installs FIVE Tools and future versions update automatically.