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FIVE Tools

MGI WashU · Bioimaging Suite

Desktop suite for plate-based bioimaging analysis — segmentation, degeneration index, and wavelength tooling. Install once; it updates itself automatically.

Windows

Windows 10/11 · 64-bit · ~518 MB
Download for Windows

macOS

Apple Silicon (arm64) · macOS 14+ · ~666 MB
Download for macOS
Auto-updates: after the first install, FIVE Tools checks for new versions on launch and prompts you to update — you won't need to come back here.

Windows — first install

  1. Run the downloaded FiveAvalonia-win-Setup.exe.
  2. If Windows shows "Windows protected your PC", click More info → Run anyway (the build is unsigned but safe — it's ours).
  3. It installs to your user profile and launches. Future versions update automatically.

macOS — first install

  1. Open the downloaded .dmg and drag FiveAvalonia to Applications.
  2. First launch: right-click the app → Open → Open. If macOS says it's "damaged", clear the quarantine flag in Terminal:
    xattr -dr com.apple.quarantine /Applications/FiveAvalonia.app
  3. It then launches normally and keeps itself updated.